A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
نویسندگان
چکیده
A modular approach has been developed for enzymatic ligation of peptides/proteins with oligonucleotides through the design tag phosphoramidites as adaptors, paving way towards streamlined production peptide/protein-oligonucleotide conjugates.
منابع مشابه
Template-directed ligation of peptides to oligonucleotides.
BACKGROUND Oligonucleotide-peptide conjugates have several applications, including their potential use as therapeutic agents. We developed a strategy for the chemical ligation of unprotected peptides to oligonucleotides in aqueous solution. The two compounds are joined via a stable amide bond in a template-directed reaction. RESULTS Peptides, ending in a carboxy-terminal thioester, were conve...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2021
ISSN: ['1359-7345', '1364-548X']
DOI: https://doi.org/10.1039/d1cc01348c